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腫瘤干細胞培養基

時間:2017-12-25 15:12:33 瀏覽:

Cancer Stem Cell Medium  

PromoCell introduces as the next generation Cancer Stem Cell Medium. This medium has been designed to meet your requirements for the extended serial 3D tumorsphere culture. supports the tumorsphere/mammosphere culture of the most commonly used cancer cell lines.Setting a New Standard with Next Generation Tumorsphere Medium。

Key Advantages:

- Supports most commonly used cancer cell lines

- Allows extended serial tumorsphere passages (>10)

- High tumorsphere formation efficiency

- Chemically defined

- Serum-free and animal component free

- Ready to use

 

List of cell types tested for serial passage with the PromoCell Cancer Stem Cell Medium.

Tissue

Tested Cell Lines

Cell Lines Origin

Brain

U-87 MG

grade IV glioblastoma / astrocytoma of the human brain

Breast  

MCF-7

pleural effusion of metastatic human breast adenocarcinoma

Colon

HT-29

human colon adenocarcinoma

Connective tissue

HT1080

human fibrosarcoma

Liver

HepG2

hepatocellular carcinoma of the human liver

Lung

A-549

human lung carcinoma

Pancreas

Panc-1

epithelioid carcinoma of the human pancreatic duct

Prostate

LNCaP

lymph node metastasis of human prostate adenocarcinoma

Skin

A-431

epidermoid carcinoma of the human skin

 

The Cancer Stem Cell Culture System

Proliferation and tumorsphere formation efficiency (TFE) of MCF-7 cells during serial passage in 3D tumorsphere culture using the PromoCell Cancer Stem Cell Medium.
Robust proliferation was maintained during the culture, which was discontinued after passage 10 with no sign of growth rate inhibition. Serial passage in resulted in significant increase of TFE from 2% in P1 to 14% in P4, and 28% in P9, respectively.

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1 腫瘤干細胞培養基 382 2017-12-25
2 經典細胞培養基 ,DMEM, MCDB-1 174 2017-12-12
3 人外周血單核細胞 & T & B 細胞培養基 91 2017-12-12
4 Gemini 細胞培養用血清 145 2017-12-11
5 StemRNA? SR Reprogramming 試劑盒 42 2017-12-11
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